What are some advantages and disadvantages of the serial dilution agar plate technique (3 pts). • What volume of each dilution do you need to make in order to have enough for the replicate tests you plan to do? More tests will cover the range in more detail, but may take too long to perform (or cost too much). Now suppose you decide that six tests will be adequate (perhaps each in quadruplicate). Well, starting at 1/1,000, you need five equal dilution steps (giving you six total dilutions counting the starting 1/1,000) that end in a 20-fold higher dilution (giving 1/20,000). Fewer tests are easier to do, but may not cover the range in enough detail to get an accurate result.

Dec 8, 2016 - PS3 Game Name: One Piece: Pirate Warriors 2. Working on: CFW 4.8X ISO Region: Europe Language: English PS3 Disc ID: BLES01913.

NOTE – Download this game for ver PC (windows, Mac): One Piece Pirate Warriors 3: Join Luffy and the Straw Hat Crew on their biggest adventure yet! One Piece Pirate Warriors 3 allows you to re-live the original One Piece story from Fushia village, where everything started, to the kingdom of Dressrosa, a mysterious island dominated by the evil Donquixote Doflamingo, known as the “Heavenly Demon”. Do you have what it takes to defeat the most dangerous pirates, including Doflamingo and many more? Gather every member of the Straw Hat Crew and sail to the most emblematic places and enter the most epic battles: Marineford, Fishman Island, Punk Hazard and Dressrosa! I am so happy when you connect to my website.

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•: September 25, 2012 Mode(s), One Piece: Warriors is an developed by and published by for. It was released on March 1, 2012, in Japan, September 21, 2012, in Europe and September 25, 2012, in North America. The game was developed in commemoration of the 15th anniversary of the manga and anime franchise.

Pirate Warriors was also the first title in the One Piece video game franchise which was released on a PlayStation system since in 2006. As with the rest of the franchise, Pirate Warriors follows the adventures of, a young boy who accidentally eats the Gum-Gum Fruit and organizes a diverse crew of pirates called the Straw Hats to search for the titular treasure. On his journey, Luffy makes several new friends, and battles a wide variety of villains. A sequel, was released in March 2013, with a third game,, released in March 2015. Contents • • • • • • • • • • Gameplay [ ] Pirate Warriors is an action game incorporating elements from both the series and the series. The game's story mode, Main Log, allows the player to play through the storyline. The Adventure mode uses Luffy to play through the events of the One Piece series and the battle mode allows the player to use characters in battles.

In Another Log, the player can play through a character's storyline. With it are several stories exclusive to the game. Characters [ ] The game features a total of 13 playable characters. There are also numerous other who are exclusively fought with. • • • • • • • • • • • • • Similarities [ ] There are 16 chapters in Main Log mode. The cut-scenes were reused in.

Development [ ] The concept and development for Pirate Warriors began with a meeting between producer Hisashi Koinuma and producer Koji Nakajima. Nakajima read the One Piece manga and also played the video games during his youth. The producers eventually realized that an action game involving Luffy fighting with sailors in a video game would be interesting. Koinuma said that the developers wanted to 'express the charm of [original author Eiichiro] Oda's [manga] art as best as we can'.

Koinuma also wanted a 'look that worked well in the game' and to make it 'approachable while still allowing [video game] experts to put their own techniques into it.' According to Shin Unozawa, vice president of, the game's title was originally just One Piece Musou before Eiichiro Oda himself changed it.